Nakai S, Katsura K, Sugihara H, Fujita S
Dept. of Pathology, Kyoto Prefectural University of Medicine.
Gan To Kagaku Ryoho. 1995 Jun;22 Suppl 2:149-52.
We applied Fluorescence In Situ Hybridization to DNA-diploid head and neck squamous cell carcinomas to detect numerical chromosomal aberrations. Using centromeric repetitive probes of chromosomes 1, 7, 11, 17, X, and Y, we detected numerical aberrations of chromosomes in 4 of the 7 tumors examined, the main line of one tumor showed trisomy 1, while the other 3 tumors had subpopulations that showed trisomy or monosomy of 1 or 2 chromosomes examined. We found polyploidy by cytofluorometry in all 4 tumors with numerical chromosomal aberrations. These results suggest that from DNA-diploid tumors with polyploidy, subpopulation that are aneuploid at the chromosome level may arise before the ploidy of the main line shifts to over DNA-aneuploidy.
我们将荧光原位杂交技术应用于DNA二倍体头颈部鳞状细胞癌，以检测染色体数目畸变。使用染色体1、7、11、17、X和Y的着丝粒重复探针，我们在所检测的7个肿瘤中的4个中检测到了染色体数目畸变，其中一个肿瘤的主要细胞系显示三体性1，而其他3个肿瘤有亚群显示所检测的1条或2条染色体的三体性或单体性。我们通过细胞荧光测定法在所有4个有染色体数目畸变的肿瘤中发现了多倍体。这些结果表明，在具有多倍体的DNA二倍体肿瘤中，染色体水平非整倍体的亚群可能在主要细胞系的倍性转变为超过DNA非整倍体之前就已出现。
