Jara P, Delmas P, Razanamparany V, Olsen L, Dupin P, Bayol A, Bégueret J, Loison G
Sanofi Recherche, Centre de Labège, France.
J Biotechnol. 1995 Jun 1;40(2):111-20. doi: 10.1016/0168-1656(95)00036-p.
The filamentous ascomycete fungus Cryphonectria parasitica naturally secretes endothiapepsin, an aspartic proteinase. It is cultured on a commercial scale as a source of the milk-clotting enzyme for cheese making. Our objective was to increase enzyme production of an industrial C. parasitica strain by a new technique of self-cloning; it consisted in the screening for transformants producing higher levels of endothiapepsin and having integrated only the DNA fragment of interest. Such genetically improved strains that are devoid of any foreign genes should be more readily acceptable for the production of food-grade enzymes.
