交联左旋曲菌素的DNA小沟结合：观察共价连接的WPPW（沟壁-肽-肽-沟壁）基序所需的实验条件。
DNA minor groove binding of cross-linked lexitropsins: experimental conditions required to observe the covalently linked WPPW (groove wall-peptide-peptide-groove wall) motif.
作者信息
Chen Y H, Lown J W
机构信息
Department of Chemistry, University of Alberta, Edmonton, Canada.
出版信息
Biophys J. 1995 May;68(5):2041-8. doi: 10.1016/S0006-3495(95)80382-6.
Abstract
A theoretical analysis of binding interactions between covalently cross-linked lexitropsins and DNA is undertaken, in which a novel cyclic symmetric 2:2 dimeric lexitropsin-DNA-binding model is proposed. Applicability of commonly used techniques including NMR, quantitative footprinting, CD, and ethidium fluorometry to differentiate the covalently linked WPPW (groove Wall-Peptide-Peptide-groove Wall) from a 2:2 cross-linked lexitropsin-DNA duplex structure is examined.
摘要
对共价交联的亲旋菌素与DNA之间的结合相互作用进行了理论分析，其中提出了一种新型的环状对称2:2二聚体亲旋菌素-DNA结合模型。研究了常用技术（包括核磁共振、定量足迹分析、圆二色光谱和溴化乙锭荧光测定法）区分共价连接的WPPW（沟壁-肽-肽-沟壁）与2:2交联的亲旋菌素-DNA双链体结构的适用性。
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DNA minor groove binding of cross-linked lexitropsins: experimental conditions required to observe the covalently linked WPPW (groove wall-peptide-peptide-groove wall) motif.交联左旋曲菌素的DNA小沟结合：观察共价连接的WPPW（沟壁-肽-肽-沟壁）基序所需的实验条件。
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