Joudrier P, Lullien-Pellerin V, Alary R, Grosset J, Guirao A, Gautier M F
Laboratoire de Biochimie et Biologie Moléculaire des Céréales, INRA, Montpellier, France.
DNA Seq. 1995;5(3):153-62. doi: 10.3109/10425179509029355.
The CM (chloroform-methanol-soluble) proteins are low-molecular-weight cysteine-rich proteins that are found in wheat and barley endosperms. A cDNA clone encoding a Triticum durum (T. durum) CM3 protein has been isolated from a mid-maturation seed cDNA library. The T. durum CM3 protein is synthesized as a precursor including a signal peptide (SP) of 25 residues. Northern blot analysis shows that in developing seed the highest level of CM3 protein mRNA is detected at mid-maturation. The hybridization patterns obtained by Southern blot analysis indicated that T. durum CM proteins are encoded by a small multigene family. The similarity between the wheat and barley CM proteins encoded by homologous chromosomes is much higher than that between each of the three members of the T. durum family. All CM proteins contain ten cysteine residues organized in a conserved cysteine motif.
CM（氯仿-甲醇可溶性）蛋白是在小麦和大麦胚乳中发现的低分子量富含半胱氨酸的蛋白。从一个中等成熟度种子的cDNA文库中分离出了一个编码硬粒小麦（T. durum）CM3蛋白的cDNA克隆。硬粒小麦CM3蛋白以前体形式合成，包含一个25个残基的信号肽（SP）。Northern印迹分析表明，在发育中的种子中，CM3蛋白mRNA在中等成熟度时检测到的水平最高。Southern印迹分析得到的杂交模式表明，硬粒小麦CM蛋白由一个小的多基因家族编码。同源染色体编码的小麦和大麦CM蛋白之间的相似性远高于硬粒小麦家族三个成员之间的相似性。所有CM蛋白都含有以保守半胱氨酸基序排列的十个半胱氨酸残基。
