Vickers S E, Warr J R
Department of Biology, University of York, Heslington, U.K.
Cell Biol Int. 1995 Jan;19(1):35-41. doi: 10.1006/cbir.1995.1005.
We have studied the nature of P-glycoprotein gene transcripts in multidrug resistant Chinese hamster ovary cell lines selected in vincristine. Using a novel, 35S based, reverse transcription polymerase chain reaction assay, we have shown that there is a considerable increase in the level of pgp2 gene transcripts, with levels rising to a hundred fold those observed in sensitive control cells. The level of pgp1 gene transcripts was raised five fold. The levels of pgp3 gene transcripts were close to the limits of detection by the assay, but were increased in the more highly resistant cell lines. An increase in the size of the P-glycoprotein gene transcript was also observed in some of the resistant cell lines.
我们研究了长春新碱筛选的多药耐药中国仓鼠卵巢细胞系中P-糖蛋白基因转录本的性质。通过一种基于35S的新型逆转录聚合酶链反应检测方法，我们发现pgp2基因转录本水平显著增加，其水平上升至敏感对照细胞中观察到的水平的百倍。pgp1基因转录本水平提高了五倍。pgp3基因转录本水平接近该检测方法的检测极限，但在耐药性更高的细胞系中有所增加。在一些耐药细胞系中还观察到P-糖蛋白基因转录本大小的增加。
