使用氮磷检测器通过气相色谱法改进血清茶碱的测定。
Improved determination of serum theophylline by gas chromatography with use of a nitrogen-phosphorus detector.
作者信息
Vinet B, Zizian L
出版信息
Clin Chem. 1979 Jan;25(1):156-8.
PMID:761355
Abstract
We describe a method for the 7-min determination of theophylline in less than 100 muL of serum. The procedure requires no centrifugation or solvent evaporation after extraction. Butylation is done on the gas-chromatographic column by injecting the serum extract followed by a butylating mixture which contains 1-iodobutane as the alkylating agent. The method is precise, accurate, and free of interference. Results correlate well with those by ultraviolet spectrophotometry.
摘要
我们描述了一种在不到100微升血清中7分钟内测定茶碱的方法。该程序在提取后无需离心或溶剂蒸发。通过注入血清提取物，然后注入含有1-碘丁烷作为烷基化剂的丁基化混合物，在气相色谱柱上进行丁基化。该方法精确、准确且无干扰。结果与紫外分光光度法的结果相关性良好。
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