纯化某些放射性标记糖肽激素的改进方法。
Improved method of purifying some radiolabeled glycopeptide hormones.
作者信息
Patritti-Laborde N, Yoshimoto Y, Wolfsen A, Odell W D
出版信息
Clin Chem. 1979 Jan;25(1):163-5.
PMID:761358
Abstract
We describe a simplified method for purifying radiolabeled glycopeptide hormones (thyrotropin, lutropin, follitropin, and human choriogonadotropin), which results in preparations of over 95% immunoreactivity. This method is based on competitive elution from columns of the plant lectin, concanavalin A, and is applicable to glycopeptide hormones derived from several species.
摘要
我们描述了一种纯化放射性标记糖肽激素（促甲状腺激素、促黄体生成素、促卵泡激素和人绒毛膜促性腺激素）的简化方法，该方法可得到免疫反应性超过95%的制剂。此方法基于从植物凝集素刀豆球蛋白A柱上进行竞争性洗脱，适用于源自多个物种的糖肽激素。
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