Raisys V A, Zebelman A M, MacMillan S F
Clin Chem. 1979 Jan;25(1):172-5.
We describe a gas-liquid chromatographic method for determining mephenytoin and its active metabolite, desmethylmephenytoin, in human serum. 5-Methyl-5-phenylhydantoin is used as the internal standard. The method involves extraction of the drugs by adsorption onto charcoal and off-column derivatization to their pentyl derivatives. Peak height and concentration are linearly related and the day-to-day CV for therapeutic concentration is about 2 to 6%. No interferences by endogenous compounds or drugs commonly used for seizure control have been encountered.
我们描述了一种用于测定人血清中美芬妥英及其活性代谢物去甲美芬妥英的气相色谱法。5-甲基-5-苯基乙内酰脲用作内标。该方法包括通过吸附到活性炭上提取药物，并进行柱外衍生化生成其戊基衍生物。峰高与浓度呈线性关系，治疗浓度的日间变异系数约为2%至6%。未遇到内源性化合物或常用于控制癫痫发作的药物的干扰。
