Weiler E W, Zenk M H
Clin Chem. 1979 Jan;25(1):44-7.
We report the synthesis and properties of a new digoxin derivative, prepared by reacting cyanogen bromide-activated digoxin with tyramine. The derivative is extremely stable, and its monoiodinated form can be isolated in 85--90% purity by a single thin-layer chromatographic step. [125I]Tyramine-digoxin is immunologically stable for at least four months (immunoreactivities, 90% or greater throughout), with a shelf life of about six months. Nonspecific binding of less than 10% was observed for several batches during this time.
我们报道了一种新的地高辛衍生物的合成及其性质，该衍生物是通过溴化氰活化的地高辛与酪胺反应制备而成。该衍生物极其稳定，其单碘化形式可通过单次薄层色谱步骤分离得到纯度为85%至90%的产物。[125I]酪胺 - 地高辛在免疫方面至少四个月保持稳定（在此期间免疫活性始终保持在90%或更高），保质期约为六个月。在此期间，对几批产品的非特异性结合观察到均小于10%。
