Depolarization effects on the peptidergic phenotypes of chick sympathetic and adrenal cells.

Depolarizing stimuli are among the factors known to influence the phenotypic plasticity of nerve cells. In order to determine the prevalence of the depolarization effects in terms of cell and neuropeptide phenotypes, we have analyzed the effect of potassium (K+)-induced depolarization on the avian sympathoadrenal system. The expression of three peptidergic phenotypes, somatostatin (SS), neuropeptide Y (NPY) and enkephalin (Enk) by two cell types, adrenal and sympathetic, was studied under different depolarizing regimens. Cells from the sympathetic paravertebral ganglion and adrenal gland of 10-11-day chick embryos were cultured and the peptide levels were measured by radioimmunoassays. Chronic depolarization causes differential effects on the peptidergic phenotypes increasing NPY and Enk but decreasing SS in both adrenal and sympathetic cultures. However, shorter exposures to depolarizing stimuli revealed diverse effects on NPY and Enk phenotypes and even between adrenal and sympathetic cells. Moreover, the maintenance of the effects after removal of the depolarizing stimuli showed additional differences among the phenotypes. Our results are not compatible with a previously established hypothesis stating that depolarization increases the synthesis of whichever neurotransmitters a neuron is already producing. They provide evidence indicating that the depolarization effect is much more complex than originally thought, and serve to initiate an in depth probe into the effect of depolarization of cellular plasticity.