Veelaert D, Schoofs L, Spittaels K, Tips A, de Loof A
Zoological Institute, Katholieke Universiteit Leuven, Belgium.
Comp Biochem Physiol A Physiol. 1995 Jul;111(3):461-67. doi: 10.1016/0300-9629(95)00027-5.
A polyclonal antibody raised against locustamyoinhibin (Lom-MIH), a myoinhibiting neuropeptide of the locust Locusta migratoria, was used to search for locustamyoinhibin-like immunoreactivity in the central nervous system of the gray fleshfly, Neobellieria bullata, the Colorado potato beetle, Leptinotarsa decemlineata, the cabbage moth, Mamestra brassicae and the cockroach, Leucophaea maderae. In L. maderea, immunoreactive cells are present in the pars intercerebralis (PI), in nerve fibers leading to the corpus cardiacum (CC) and in the CC themselves. In N. bullata, three groups of cells are positive: one in the PI, one in the pars lateralis and one in the suboesophageal ganglion. In M. brassicae, there are only positive cells in the PI. No immunoreactivity was found in L. decemlineata. These results indicate that the presence of Lom-MIH immuno-like molecules is not restricted to the orthopterans, and that they can be localized in different parts of the head ganglia.
一种针对飞蝗肌抑制素（Lom-MIH）产生的多克隆抗体被用于在灰肉蝇（Neobellieria bullata）、科罗拉多马铃薯甲虫（Leptinotarsa decemlineata）、甘蓝夜蛾（Mamestra brassicae）和蟑螂（Leucophaea maderae）的中枢神经系统中寻找类似飞蝗肌抑制素的免疫反应性。在马德拉蜚蠊（L. maderae）中，免疫反应性细胞存在于脑间部（PI）、通向心侧体（CC）的神经纤维以及心侧体本身。在灰肉蝇（N. bullata）中，有三组细胞呈阳性：一组在脑间部，一组在外侧部，一组在咽下神经节。在甘蓝夜蛾（M. brassicae）中，只有脑间部存在阳性细胞。在科罗拉多马铃薯甲虫（L. decemlineata）中未发现免疫反应性。这些结果表明，类似飞蝗肌抑制素的免疫样分子不仅存在于直翅目昆虫中，而且可以定位在头部神经节的不同部位。
