Nomura H, Sato H, Seiki M, Mai M, Okada Y
Department of Molecular Immunology and Pathology, Kanazawa University, Ishikawa, Japan.
Cancer Res. 1995 Aug 1;55(15):3263-6.
We have examined the implications of membrane-type matrix metalloproteinase (MT-MMP) for activation of the zymogen of MMP-2 (proMMP-2) in human gastric carcinoma. Northern blot analysis demonstrated exclusive expression of MT-MMP in the carcinoma tissues. Immunohistochemically, MT-MMP was colocalized in the carcinoma cells in almost all MMP-2-positive cases (13 of 14 cases). Gelatin zymography of the culture media showed a correlation of the proMMP-2 activation with MT-MMP expression in the carcinoma cells. A microdissection study indicated that proMMP-2 activation is caused only in the carcinoma cell nests that express MT-MMP but not in the normal gastric mucosa. These results are the first demonstration of the MT-MMP-assisted activation of proMMP-2 in the human gastric carcinoma.
我们研究了膜型基质金属蛋白酶（MT-MMP）在人胃癌中对MMP-2酶原（proMMP-2）激活的影响。Northern印迹分析表明MT-MMP在癌组织中特异性表达。免疫组织化学显示，在几乎所有MMP-2阳性病例（14例中的13例）中，MT-MMP与癌细胞共定位。培养基的明胶酶谱显示proMMP-2激活与癌细胞中MT-MMP表达相关。显微切割研究表明，proMMP-2激活仅在表达MT-MMP的癌细胞巢中发生，而在正常胃黏膜中不发生。这些结果首次证明了MT-MMP在人胃癌中对proMMP-2的辅助激活作用。
