Kappe A L, van de Zande L, Vedder E J, Bijlsma R, van Delden W
Department of Genetics, University of Groningen, Haren, The Netherlands.
Heredity (Edinb). 1995 Jun;74 ( Pt 6):647-53. doi: 10.1038/hdy.1995.88.
Genetic variation in two harbour seal (Phoca vitulina) populations from the Dutch Wadden Sea and Scotland was examined by RAPD analysis and DNA fingerprinting. For comparison a population of grey seals (Halichoerus grypus) was studied. The RAPD method revealed a very low number of polymorphic bands. The multilocus DNA fingerprinting technique showed very low levels of variation both within and between populations of the harbour seals. For one probe a significant difference in variation between populations was demonstrated. The estimated average heterozygosity, however, diverged more strongly between the two harbour seal populations because of more frequent occurrence of rare alleles in the Scottish population. Low levels of genetic variation seem to be characteristic for the subspecies Phoca vitulina vitulina, but not for all marine mammals in the North Sea, as the grey seal population showed significantly higher variation.
通过随机扩增多态性DNA（RAPD）分析和DNA指纹技术，对来自荷兰瓦登海和苏格兰的两个港海豹（Phoca vitulina）种群的遗传变异进行了研究。为作比较，还研究了一个灰海豹（Halichoerus grypus）种群。RAPD方法显示多态性条带数量非常少。多位点DNA指纹技术表明，港海豹种群内部和种群之间的变异水平都非常低。对于一个探针，证明了种群之间变异存在显著差异。然而，由于苏格兰种群中稀有等位基因出现频率更高，两个港海豹种群之间估计的平均杂合度差异更为明显。低水平的遗传变异似乎是港海豹亚种Phoca vitulina vitulina的特征，但并非北海所有海洋哺乳动物的特征，因为灰海豹种群显示出显著更高的变异水平。
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