Parker M L, Brocklehurst T F, Gunning P A, Coleman H P, Robins M M
Institute of Food Research, Norwich Laboratory, Colney, UK.
J Appl Bacteriol. 1995 Jun;78(6):601-8. doi: 10.1111/j.1365-2672.1995.tb03105.x.
Methods are presented for investigating the site and form of growth of bacteria in model oil-in-water emulsions and in dairy cream. Following growth of the bacteria, the continuous aqueous phase is gelled using agarose and the oil phase removed using a mixture of chloroform and methanol. Using this method, the authors have found that Listeria monocytogenes, Salmonella typhimurium and Yersinia enterocolitica grow in the form of colonies in concentrated oil-in-water emulsions. Colonies of L. monocytogenes and Y. enterocolitica also form in artificially-inoculated fresh and tinned dairy cream. If information about the precise site of growth is not required, the authors have discovered that intact colonies can be liberated from the model emulsions by dissolving away the oil phase with chloroform:methanol.
本文介绍了用于研究细菌在水包油型乳液模型和乳脂中生长位点及生长形式的方法。细菌生长后，使用琼脂糖使连续水相凝胶化，并使用氯仿和甲醇的混合物去除油相。通过这种方法，作者发现单核细胞增生李斯特菌、鼠伤寒沙门氏菌和小肠结肠炎耶尔森氏菌在浓缩水包油型乳液中以菌落形式生长。单核细胞增生李斯特菌和小肠结肠炎耶尔森氏菌的菌落在人工接种的新鲜和罐装乳脂中也会形成。如果不需要关于精确生长位点的信息，作者发现用氯仿：甲醇溶解油相可以从模型乳液中释放完整的菌落。
