Taniguchi Y
Fourth Department of Internal Medicine, Nippon Medical School, Tokyo, Japan.
Nihon Ika Daigaku Zasshi. 1995 Jun;62(3):241-50. doi: 10.1272/jnms1923.62.241.
To study the relation between p53 mutation and metastasis in primary lung cancer, 29 pairs of primary and metastatic tumors obtained by autopsy were analyzed for abnormalities of the p53 gene, by the polymerase chain reaction-single strand conformation polymorphism (PCR-SSCP). The tumors consisted of 6 small cell carcinomas, 13 adenocarcinomas, 8 squamous cell carcinomas, 1 large cell carcinoma, and 1 adeno-squamous cell carcinoma. PCR-SSCP analysis showed that 3 small cell carcinomas (50%), 3 adenocarcinomas (23%), 2 squamous cell carcinomas (25%), and 1 large cell carcinoma (100%) had p53 gene mutations. The abnormalities were found between exons five and eight. The metastatic tumor and the primary tumor had similar mutations. These results suggest that p53 gene mutation may occur before distant metastasis and may be stable during the process of metastasis.
为研究p53基因突变与原发性肺癌转移之间的关系，采用聚合酶链反应-单链构象多态性（PCR-SSCP）方法，对29对经尸检获得的原发性和转移性肿瘤进行p53基因异常分析。这些肿瘤包括6例小细胞癌、13例腺癌、8例鳞状细胞癌、1例大细胞癌和1例腺鳞癌。PCR-SSCP分析显示，3例小细胞癌（50%）、3例腺癌（23%）、2例鳞状细胞癌（25%）和1例大细胞癌（100%）存在p53基因突变。异常发生在第5至8外显子之间。转移瘤和原发瘤具有相似的突变。这些结果表明，p53基因突变可能在远处转移之前就已发生，并且在转移过程中可能是稳定的。
