通过小型十二烷基硫酸钠聚丙烯酰胺凝胶电泳快速、安全地测定非胰岛素依赖型糖尿病患者的人类载脂蛋白E基因型
Rapid and safe determination of human apolipoprotein E genotypes by miniaturised SDS-PAGE in non-insulin dependent diabetes mellitus.
作者信息
Clavel C, Durlach A, Durlach V, Birembaut P
机构信息
Unité de Biologie Cellulaire, Laboratoire Pol Bouin, Hôpital Maison Blanche, Reims, France.
出版信息
J Clin Pathol. 1995 Apr;48(4):295-9. doi: 10.1136/jcp.48.4.295.
AIMS
To present a non-isotopic procedure for the analysis of the different apolipoprotein E genotypes in normal subjects and patients with non-insulin dependent diabetes mellitus.
METHODS
Apolipoprotein E genotypes were detected following polymerase chain reaction and miniaturised sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE) (PhastSystem Pharmacia).
RESULTS
The time taken from extraction of DNA from 6 microliters whole blood to the final result was eight hours. The allele frequencies in patients with diabetes mellitus in our series were similar to those found in the control subjects.
CONCLUSIONS
SDS-PAGE is rapid, reliable and safe with few drawbacks, can be used as a routine procedure and may easily replace apolipoprotein E phenotyping.
目的
介绍一种用于分析正常受试者和非胰岛素依赖型糖尿病患者不同载脂蛋白E基因型的非同位素方法。
方法
采用聚合酶链反应和小型化十二烷基硫酸钠聚丙烯酰胺凝胶电泳（SDS-PAGE）（Pharmacia公司的PhastSystem）检测载脂蛋白E基因型。
结果
从6微升全血中提取DNA到得出最终结果所需时间为8小时。我们研究系列中的糖尿病患者的等位基因频率与对照组相似。
结论
SDS-PAGE快速、可靠且安全，缺点少，可作为常规方法使用，并且可能很容易取代载脂蛋白E表型分析。
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