Huang P, Allam A, Taghian A, Freeman J, Duffy M, Suit H D
Edwin L. Steele Laboratory of Radiation Biology, Department of Radiation Oncology, Massachusetts General Hospital, Harvard Medical School, Boston, USA.
J Neurosurg. 1995 Aug;83(2):308-15. doi: 10.3171/jns.1995.83.2.0308.
The growth and metastatic behavior of five human glioblastoma multiforme xenografts and nine human xenografts of various histological types were compared in severe combined immunodeficient (SCID) mice. The results demonstrate that the metastatic behavior of the human glioblastoma multiforme xenografts did not differ significantly from a variety of other histological xenografts when evaluated at the same transplantation site in the SCID model. These results are consistent with the hypothesis that the site of glioblastoma multiforme growth influences the extraneural metastatic spread of this disease and lead the authors to suggest that the clinical rarity of distant metastasis is not a fundamental property of these cells. A total of 340 male 7- to 8-week-old SCID mice received subcutaneous transplantation of tumor fragments (21-25 mice per tumor type). The tumor-bearing leg was amputated when the tumor reached a volume of 500 mm3; mice were observed for up to 5 months. There was a trend for a lower take rate, longer latent period, longer volume doubling time (VDT) and growth time (GT) in glioblastoma multiforme as opposed to carcinoma and soft tissue sarcoma xenografts. The highest local recurrence rates (78% and 68%) were observed in two glioblastomas multiforme. Both the glioblastoma multiforme and the other histological xenografts exhibited a widely varying metastatic rate: no correlation was demonstrated between VDT, GT, local control/recurrence, and distant metastasis. These findings show SCID mice to be an attractive model for further biological and preclinical studies of human glioblastoma multiforme.
在严重联合免疫缺陷（SCID）小鼠中比较了5种多形性胶质母细胞瘤人源异种移植瘤和9种不同组织学类型的人源异种移植瘤的生长和转移行为。结果表明，在SCID模型的同一移植部位进行评估时，多形性胶质母细胞瘤人源异种移植瘤的转移行为与其他多种组织学异种移植瘤没有显著差异。这些结果与以下假设一致，即多形性胶质母细胞瘤的生长部位会影响该疾病的神经外转移扩散，并使作者提出远处转移在临床上罕见并非这些细胞的基本特性。总共340只7至8周龄的雄性SCID小鼠接受了肿瘤片段的皮下移植（每种肿瘤类型21 - 25只小鼠）。当肿瘤体积达到500立方毫米时，将荷瘤腿截肢；对小鼠观察长达5个月。与癌和软组织肉瘤异种移植瘤相比，多形性胶质母细胞瘤的接种率较低、潜伏期较长、体积倍增时间（VDT）和生长时间（GT）较长。在两种多形性胶质母细胞瘤中观察到最高的局部复发率（分别为78%和68%）。多形性胶质母细胞瘤和其他组织学异种移植瘤的转移率都有很大差异：VDT、GT、局部控制/复发与远处转移之间未显示出相关性。这些发现表明SCID小鼠是用于人类多形性胶质母细胞瘤进一步生物学和临床前研究的有吸引力的模型。
