Yeung C H, Oberländer G, Cooper T G
Institute of Reproductive Medicine of the University, Münster, Germany.
J Reprod Fertil. 1995 Mar;103(2):257-64. doi: 10.1530/jrf.0.1030257.
Infertility is induced in male rats by oral administration of 400 mg ornidazole kg-1 body mass day-1 within 10 days, without drastic effect on the motility of epididymal spermatozoa obtained after 15 days of treatment. Spermatozoa were recovered from the female tract 9 h after mating with males treated with ornidazole for 10 days to identify the cause of infertility. The number and motility of spermatozoa in the uterus indicated normal seminal deposition. Spermatozoa could pass the utero-tubal junction but total numbers of spermatozoa and their velocities in the oviductal isthmus were significantly lower than those recovered from females mated to fertile, control males fed vehicle alone. However, the number of spermatozoa recovered from the ampulla was not different from control and the number of spermatozoa that had penetrated the cumulus mass was lower and none of the eggs was fertilized by spermatozoa from rats treated with ornidazole. The ability of cauda epididymal spermatozoa from ornidazole-fed rats to penetrate viscous media in vitro was lower than that of the controls. When incubated in media containing different concentrations of substrates and ions, a reduction in movement of spermatozoa was observed when glucose was the only exogenous substrate or after preincubation in substrate-free medium. These results indicate that the antifertility action of orally administered ornidazole occurs via damage to spermatozoa in the upper regions of the female tract, possibly reflecting a failure in capacitation as a result of reduced energy production.
通过以400毫克奥硝唑/千克体重/天的剂量连续10天口服给药，可诱导雄性大鼠不育，且对治疗15天后获得的附睾精子活力没有显著影响。在与经奥硝唑处理10天的雄性大鼠交配9小时后，从雌性生殖道中回收精子，以确定不育的原因。子宫内精子的数量和活力表明精液沉积正常。精子能够通过子宫输卵管连接部，但输卵管峡部的精子总数及其速度显著低于与仅喂食赋形剂的可育对照雄性大鼠交配的雌性大鼠所回收的精子。然而，从壶腹部回收的精子数量与对照组无差异，穿透卵丘团块的精子数量较少，且用奥硝唑处理的大鼠的精子没有使任何卵子受精。经奥硝唑喂养的大鼠附睾尾部精子体外穿透粘性介质的能力低于对照组。当在含有不同浓度底物和离子的培养基中孵育时，当葡萄糖是唯一的外源底物或在无底物培养基中预孵育后，观察到精子运动减少。这些结果表明，口服奥硝唑的抗生育作用是通过损伤雌性生殖道上部的精子而发生的，这可能反映了由于能量产生减少导致的获能失败。
