Otsu M, Hayashi Y, Amatsu M, Itoh H
Department of Otorhinolaryngology-Head and Neck Surgery, Kobe University School of Medicine.
Kobe J Med Sci. 1994 Dec;40(5-6):139-53.
We have characterized 24 hypopharyngeal squamous cell carcinomas and 5 normal hypopharyngeal tissues by immunostaining with antibodies against epidermal growth factor (EGF), EGF-Receptor (EGFR), p53, v-erb B, and ras p21. The Avidin-Biotin Complex (ABC) technique was employed. Overexpression of p53 appeared in 17 of 24 cases of squamous cell carcinoma of the hypopharynx (normal mucosa: none, well differentiated: 60%, moderately differentiated: 71.4%, poorly differentiated: 71.4%). Some dysplastic mucosae surrounding cancer lesions showed overexpression of p53. EGF and EGFR tended to be expressed more strongly in carcinoma [EGF: 29.1% (well differentiated: 30%, moderately differentiated: 28.6%, poorly differentiated: 28.6%); EGFR: 50% (well differentiated: 60%, moderately differentiated: 42.9%, poorly differentiated: 42.9%)] than in normal mucosa (EGF: 0%, EGFR: 20%). The v-erb B stained positively in carcinoma [62.5% (well differentiated: 70%, moderately differentiated: 71.4%, poorly differentiated: 42.9%)] but negatively in normal mucosa. These data suggest that genetic mutations of p53 probably play an important role at an early stage of tumorigenesis, and that the networks of EGF, EGFR and v-erb B probably are involved in the development of hypopharyngeal squamous cell carcinoma.
我们通过用抗表皮生长因子（EGF）、表皮生长因子受体（EGFR）、p53、v-erb B和ras p21的抗体进行免疫染色，对24例下咽鳞状细胞癌和5例正常下咽组织进行了特征分析。采用了抗生物素蛋白-生物素复合物（ABC）技术。p53的过表达出现在24例下咽鳞状细胞癌中的17例（正常黏膜：无，高分化：60%，中分化：71.4%，低分化：71.4%）。癌灶周围的一些发育异常黏膜显示p53过表达。EGF和EGFR在癌组织中往往比在正常黏膜中表达更强[EGF：29.1%（高分化：30%，中分化：28.6%，低分化：28.6%）；EGFR：50%（高分化：60%，中分化：42.9%，低分化：42.9%）]（EGF：0%，EGFR：20%）。v-erb B在癌组织中呈阳性染色[62.5%（高分化：70%，中分化：71.4%，低分化：42.9%）]，但在正常黏膜中呈阴性。这些数据表明，p53的基因突变可能在肿瘤发生的早期阶段起重要作用，并且EGF、EGFR和v-erb B网络可能参与了下咽鳞状细胞癌的发展。
