Mesárosová A, Hrivnáková A, Klobusická M, Babusíková O
Cancer Research Institute, Slovak Academy of Sciences, Bratislava.
Neoplasma. 1995;42(1):9-14.
Peripheral blood or bone marrow of 24 patients with chronic myeloid leukemia (CML) were characterized for their surface membrane marker profiles using flow cytometry and fluorescence microscopy. Purine metabolism enzyme activities were compared with membrane immunophenotype and cytochemical stains. CML subtypes were correlated with the expression of surface membrane antigens detected by the monoclonal antibodies. On the basis of immunophenotyping we found the following characteristic marker profiles: In stable phase of CML (CML-SP)-CD15, CD11b, CDw65, CD13, in accelerated phase of CML (CML-AP)-CD15, CDw65, CD11b, CD13 and CD33, in myeloid blastic phase of CML(CML-BP-M)-CD13, CD33, HLA-DR, CD11b, CD15, CDw65, in myeloid and lymphoid (mixed) blastic phase of CML (CML-BP-M+L)-CD13, CD33, CD34, HLA-DR, CD11b, CD10 and in chronic myelomonocytic leukemia (CMML)-CD14, CDw65, CD11b, CD33 and HLA-DR. Analysis of purine metabolism enzyme activities showed that there was a correlation between the values of adenosine deaminase (ADA) and purine nucleoside phosphorylase (PNP) and various types of CML. ADA levels in CML-SP, CML-AP and CMML were comparable with those in normal cells. In CML-BP-M, which represents proliferation of less mature myeloid cells (similar to less mature AML subtypes), ADA activity increased and PNP activity decreased. ADA activity was significantly different between control group and CML-BP-M (p < 0.01), between CML-SP and CML-BP-M (p < 0.05). The values of PNP activity were the highest in stable phase of CML (125 pkat. 10(-6) cells) and the lowest (23 pkat.10(-6) cells) in CML-BP-M+L. PNP activity in the other groups corresponded to control values. High ADA/PNP ratio was found in CML-BP-M and CML-BP-M+L (0.7 and 2.0, respectively) in comparison to CML-SP (0.2). It follows from our results that ADA/PNP ratio enables to discriminate between stable and blast phases of CML (p < 0.01). The level of the cytochemical enzymes (CHAE, MPO, SBB, ANAE and 5' NT) varied and reflected the degree of cell differentiation and maturation. CHAE and MPO were characteristic enzymes for CML, ANBE for CMML and 5' NT for CML-BP-lymphoid.
采用流式细胞术和荧光显微镜对24例慢性髓性白血病（CML）患者的外周血或骨髓进行表面膜标志物谱分析。将嘌呤代谢酶活性与膜免疫表型和细胞化学染色进行比较。CML亚型与单克隆抗体检测到的表面膜抗原表达相关。基于免疫表型分析，我们发现了以下特征性标志物谱：在CML稳定期（CML-SP）为CD15、CD11b、CDw65、CD13；在CML加速期（CML-AP）为CD15、CDw65、CD11b、CD13和CD33；在CML髓系原始细胞期（CML-BP-M）为CD13、CD33、HLA-DR、CD11b、CD15、CDw65；在CML髓系和淋巴系（混合）原始细胞期（CML-BP-M+L）为CD13、CD33、CD34、HLA-DR、CD11b、CD10；在慢性粒单核细胞白血病（CMML）为CD14、CDw65、CD11b、CD33和HLA-DR。嘌呤代谢酶活性分析表明，腺苷脱氨酶（ADA）和嘌呤核苷磷酸化酶（PNP）的值与各种类型的CML之间存在相关性。CML-SP、CML-AP和CMML中的ADA水平与正常细胞中的相当。在代表较不成熟髓系细胞增殖的CML-BP-M中，ADA活性增加而PNP活性降低。对照组与CML-BP-M之间（p<0.01）、CML-SP与CML-BP-M之间（p<0.05）ADA活性有显著差异。PNP活性值在CML稳定期最高（125 pkat·10⁻⁶细胞），在CML-BP-M+L中最低（23 pkat·10⁻⁶细胞）。其他组中的PNP活性与对照值相当。与CML-SP（0.2）相比，CML-BP-M和CML-BP-M+L中发现高ADA/PNP比值（分别为0.7和2.0）。从我们的结果可以看出，ADA/PNP比值能够区分CML的稳定期和原始细胞期（p<0.01）。细胞化学酶（CHAE、MPO、SBB、ANAE和5'NT）的水平各不相同，反映了细胞分化和成熟的程度。CHAE和MPO是CML的特征性酶，ANBE是CMML的特征性酶，5'NT是CML-BP-淋巴系的特征性酶。
