Gouliaev A H, Nedergaard O A
Department of Pharmacology, School of Medicine, Odense University, Denmark.
Pharmacol Toxicol. 1995 Apr;76(4):269-77. doi: 10.1111/j.1600-0773.1995.tb00142.x.
The main aim of the present investigation was to study systematically the passive and stimulation-evoked release of 3H-5-hydroxytryptamine (3H-5-HT) from rabbit isolated aorta. This was accomplished by preloading rings of aorta with 3H-5-HT (10(-6)M) and then monitoring by fractional collection the basal 3H-outflow and stimulation-evoked 3H-overflow. The basal 3H-outflow from aorta preloaded with 10(-6)M of either 3H-5-HT or (-)-3H-noradrenaline (3H-NA) leveled off about 100 min. after the onset of wash-out and remained almost constant thereafter (100-240 min.). The basal 3H-outflow from tissue preloaded with 3H-5-HT was almost 3-fold higher (70-240 min.) than that seen after preloading with 3H-NA. Cocaine (3 x 10(-5)M) did not alter the basal 3H-outflow (15-240 min.) from tissue preloaded with 3H-5-HT, while pargyline (5 x 10(-4)M) decreased it by about 66% (100-240 min.). Electrical-field stimulation (S1-S7, 200 mA, 600 pulses, 0.5 msec., 3 Hz) were applied to the tissue. The initial stimulation-evoked 3H-overflow from aorta preloaded with 3H-5-HT was higher than the subsequent ones (S1-S7; 100, 35, 35, 35, 35, 37, and 40%). Similar results to these were obtained with tissues preloaded with 3H-NA. The stimulation (S1-S7; 200 mA). 600 pulses, 0.5 msec, 3 Hz)-evoked 3H-overflow increased in an apparent linear manner with the amount of current used (50-200 mA).(ABSTRACT TRUNCATED AT 250 WORDS)
本研究的主要目的是系统地研究兔离体主动脉中3H-5-羟色胺（3H-5-HT）的被动释放和刺激诱发释放。这是通过用3H-5-HT（10^(-6)M）预加载主动脉环，然后通过分部收集监测基础3H流出和刺激诱发的3H溢出实现的。用10^(-6)M的3H-5-HT或（-）-3H-去甲肾上腺素（3H-NA）预加载的主动脉的基础3H流出在洗脱开始后约100分钟趋于平稳，此后几乎保持恒定（100-240分钟）。用3H-5-HT预加载的组织的基础3H流出比用3H-NA预加载后高出近3倍（70-240分钟）。可卡因（3×10^(-5)M）不会改变用3H-5-HT预加载的组织的基础3H流出（15-240分钟），而帕吉林（5×10^(-4)M）使其减少约66%（100-240分钟）。对组织施加电场刺激（S1-S7，200 mA，600脉冲，0.5毫秒，3 Hz）。用3H-5-HT预加载的主动脉最初刺激诱发的3H溢出高于随后的刺激（S1-S7；100%、35%、35%、35%、35%、37%和40%）。用3H-NA预加载的组织也得到了类似的结果。刺激（S1-S7；200 mA，600脉冲，0.5毫秒，3 Hz）诱发的3H溢出随着所用电流量（50-200 mA）以明显的线性方式增加。（摘要截断于250字）
