Brown E, Lloyd L, Sheth A, Oweis T, Chen J C, Penman L, Mattson J C, Steel K
Department of Surgery, St. John Hospital and Medical Center, Detroit, Michigan 48236-2172, USA.
Am Surg. 1995 Aug;61(8):669-72; discussion 672-3.
The purpose of this study is to examine the effects of three different types of fluid resuscitation on the immune system of dogs in hemorrhagic shock. Using a modified Wigger shock model, 18 conditioned male dogs were bled to mean arterial blood pressure of 60 mm Hg for 90 minutes and placed into three groups based on the resuscitative method. Group I: Crystalloid Resuscitation; Group II: Autotransfusion; Group III: Banked Blood. Laboratory methods for immune status evaluation included total lymphocyte count, T4/T8 ratio, total serum immunoglobulins, and immunoglobulin electrophoresis. These values were obtained pre-hemorrhagic shock, just before resuscitation, and subsequently on days 1, 4, and 7. Humoral immunity, represented by total serum immunoglobulin levels (IgA, IgG, IgM), was higher in Groups II and III when compared with group I on all post-resuscitation days. IgA and IgM levels were higher in Group III compared with Groups I and II. IgG level was higher in Group II compared with Groups I and III. Cellular immunity was also affected by transfusion. Total lymphocyte count was increased in Group II on Day 1; however, the three groups were similar with respect to this variable on subsequent days. The absolute T4 helper cell level in Group II was similar to Groups I and III until Day 7, at which time the level became higher in Group II.(ABSTRACT TRUNCATED AT 250 WORDS)
本研究的目的是探讨三种不同类型的液体复苏对失血性休克犬免疫系统的影响。采用改良的维格休克模型，将18只条件性雄性犬放血至平均动脉血压60 mmHg，持续90分钟，并根据复苏方法分为三组。第一组：晶体液复苏；第二组：自体输血；第三组：库存血。评估免疫状态的实验室方法包括总淋巴细胞计数、T4/T8比值、血清总免疫球蛋白和免疫球蛋白电泳。这些数值在出血性休克前、复苏前以及随后的第1、4和7天获取。以血清总免疫球蛋白水平（IgA、IgG、IgM）为代表的体液免疫，在复苏后的所有天数中，第二组和第三组均高于第一组。与第一组和第二组相比，第三组的IgA和IgM水平更高。与第一组和第三组相比。第二组的IgG水平更高。细胞免疫也受到输血的影响。第一组在第1天总淋巴细胞计数增加；然而，在随后的日子里，三组在这个变量上相似。直到第7天，第二组的绝对T4辅助细胞水平与第一组和第三组相似，此时第二组的水平变得更高。（摘要截短为250字）
