从肉类中分离的产细菌素乳酸杆菌中乳链菌肽S结构基因的PCR检测
PCR detection of the lactocin S structural gene in bacteriocin-producing lactobacilli from meat.
作者信息
Rodríguez J M, Cintas L M, Casaus P, Suárez A, Hernández P E
机构信息
Departamento de Nutrición y Bromatología III, Facultad de Veterinaria, Universidad Complutense, Madrid, Spain.
出版信息
Appl Environ Microbiol. 1995 Jul;61(7):2802-5. doi: 10.1128/aem.61.7.2802-2805.1995.
Abstract
The lactocin S structural gene (lasA) in seven bacteriocinogenic lactobacilli isolated from fermented sausages was studied. Two degenerate primers were synthesized to amplify a 75-bp fragment of the gene. Three strains amplified the fragment from their plasmid DNA, and hybridization analysis confirmed these results.
摘要
对从发酵香肠中分离出的7株产细菌素的乳酸杆菌中的乳酸链球菌素S结构基因（lasA）进行了研究。合成了两条简并引物，以扩增该基因的一个75 bp片段。三株菌株从其质粒DNA中扩增出该片段，杂交分析证实了这些结果。
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