用于检测感染和接种布鲁氏菌的绵羊的酶联免疫吸附测定法的评估。

Evaluation of an enzyme-linked immunosorbent assay for the detection of sheep infected and vaccinated with Brucella melitensis.

作者信息

Delgado S, Fernández M, Cármenes P

机构信息

Departamento de Sanidad Animal (Enfermedades Infecciosas y Epidemiología), Facultad de Veterinaria, Universidad de León, Spain.

出版信息

J Vet Diagn Invest. 1995 Apr;7(2):206-9. doi: 10.1177/104063879500700207.

DOI:10.1177/104063879500700207

PMID:7619903

Abstract

An indirect enzyme-linked immunosorbent assay (ELISA), using the lipopolysaccharide of the cell wall as an antigen, was used to detect Brucella melitensis antibodies in ovine serum. The test was carried out on 703 samples of field serums, which were also analyzed by the complement fixation (CF) test and the rose Bengal (RB) test. The ELISA results were more similar to those of the CF test (kappa = 0.89) than to the results of the RB test (kappa = 0.73). The ELISA also had high sensitivity (94.7%) and a somewhat lower specificity (90.4%). One group of 139 young brucellosis-free animals 3-6 months of age were vaccinated with B. melitensis rev. 1 at a dose of 1.2 x 10(9) live organisms. The ELISA detected a significantly lower number of reactors than the CF and RB tests (P < 0.001). The ELISA values remained below the cutoff level during the 9 months following vaccination.

摘要

采用以细胞壁脂多糖为抗原的间接酶联免疫吸附测定（ELISA）法检测绵羊血清中的羊布鲁氏菌抗体。对703份现场血清样本进行了该检测，同时这些样本也通过补体结合（CF）试验和玫瑰红（RB）试验进行了分析。ELISA结果与CF试验结果（kappa = 0.89）的相似性高于与RB试验结果（kappa = 0.73）的相似性。ELISA还具有较高的敏感性（94.7%）和稍低的特异性（90.4%）。一组139只3至6月龄无布鲁氏菌病的幼龄动物接种了剂量为1.2×10⁹活菌体的羊布鲁氏菌Rev. 1疫苗。ELISA检测出的反应动物数量显著低于CF试验和RB试验（P < 0.001）。接种疫苗后的9个月内，ELISA值一直低于临界值水平。