Ottaviani E, Capriglione T, Franceschi C
Department of Animal Biology, University of Modena, Italy.
Brain Behav Immun. 1995 Mar;9(1):1-8. doi: 10.1006/brbi.1995.1001.
We show here that hemocytes and leukocytes with phagocytic activity from both invertebrates (Planorbarius corneus, Viviparus ater) and vertebrates (Carassius auratus, Rana esculenta) express pro-opiomelanocortin (POMC) mRNA, as assessed by in situ hybridization with a digoxigenin-labeled human DNA probe. These data are in accord with previous observations from our laboratories on the presence in these cells of POMC-derived peptides and strongly suggest that these molecules--highly conserved throughout evolution--play an important role in cell locomotion and phagocytosis. POMC mRNA was also detected in lymphocytes of R. esculenta, but not of C. auratus, suggesting that from anuran amphibians onwards lymphocytes also express this gene. This phenomenon could be related to the appearance of more than one immunoglobulin isotype in anurans.
我们在此表明，通过用地高辛标记的人类DNA探针进行原位杂交评估，来自无脊椎动物（角扁卷螺、乌苏里圆田螺）和脊椎动物（鲫鱼、食用蛙）的具有吞噬活性的血细胞和白细胞表达促肾上腺皮质激素原（POMC）mRNA。这些数据与我们实验室之前关于这些细胞中存在POMC衍生肽的观察结果一致，并强烈表明这些在整个进化过程中高度保守的分子在细胞运动和吞噬作用中发挥重要作用。在食用蛙的淋巴细胞中也检测到了POMC mRNA，但鲫鱼的淋巴细胞中未检测到，这表明从无尾两栖动物开始，淋巴细胞也表达该基因。这种现象可能与无尾两栖动物中出现多种免疫球蛋白同种型有关。
