Fujimaki H, Nohara O, Katayama N, Abe T, Nohara K
National Institute for Environmental Studies, Ibaraki, Japan.
Int Arch Allergy Immunol. 1995 Aug;107(4):527-32. doi: 10.1159/000237095.
To investigate the modulated proliferation of an interleukin-3(IL-3)-dependent cell by exogenous ganglioside GM3, mouse bone marrow-derived mast cells (BMMC) were cultured with various concentrations of GM3 in the presence of IL-3. By 4 weeks of culture, most of the nonadherent cells were alcian blue-positive mast cells. Culturing 2-week-cultured BMMC with GM3 for 1 week reduced the number of alcian blue-positive cells, but the increased total histamine content of BMMC was observed. To examine the effect of GM3 on the synergistic response by IL-3 and interleukin-4 (IL-4), 3-week-cultured BMMC were cultured with GM3 in the presence of IL-3 and IL-4 for 1 week. Although the addition of IL-4 to culture medium increased the number of BMMC, treatment with GM3 reduced its proliferative activity. Concerning the effect of GM3 on cell membrane, there are no changes in the expression of IgE receptors on BMMC treated with GM3 though a low concentration of GM3 increased it. However, the production of tumor necrosis factor-alpha from BMMC treated with GM3 was significantly suppressed. These results indicate that in vitro treatment with exogenous GM3 inhibited the proliferative response of IL-3-dependent mast cell populations and modulated its characteristics.
为研究外源性神经节苷脂GM3对白细胞介素-3（IL-3）依赖细胞增殖的调节作用，将小鼠骨髓来源的肥大细胞（BMMC）在IL-3存在的情况下与不同浓度的GM3一起培养。培养4周后，大多数非贴壁细胞为阿尔辛蓝阳性肥大细胞。将培养2周的BMMC与GM3一起培养1周可减少阿尔辛蓝阳性细胞的数量，但观察到BMMC的总组胺含量增加。为研究GM3对IL-3和白细胞介素-4（IL-4）协同反应的影响，将培养3周的BMMC在IL-3和IL-4存在的情况下与GM3一起培养1周。虽然向培养基中添加IL-4可增加BMMC的数量，但用GM3处理可降低其增殖活性。关于GM3对细胞膜的影响，用GM3处理的BMMC上IgE受体的表达没有变化，尽管低浓度的GM3可增加其表达。然而，用GM3处理的BMMC中肿瘤坏死因子-α的产生受到显著抑制。这些结果表明，体外使用外源性GM3处理可抑制IL-3依赖的肥大细胞群体的增殖反应并调节其特性。
