胶束高效液相色谱法分析大肠杆菌发酵液中的重组人生长激素
Analysis of recombinant human growth hormone in Escherichia coli fermentation broth by micellar high-performance liquid chromatography.
作者信息
Strege M A, Lagu A L
机构信息
Lilly Research Laboratories, Division of Eli Lilly and Co., Indianapolis, IN 46285, USA.
出版信息
J Chromatogr A. 1995 Jun 23;705(1):155-61. doi: 10.1016/0021-9673(94)01253-b.
A method for the reversed-phase high-performance liquid chromatographic (HPLC) determination of recombinant methionylaspartyl-human growth hormone (MD-HGH) in Escherichia coli fermentation broth is described. The technique utilizes mobile phases containing n-propanol and the anionic surfactant sodium dodecyl sulfate (SDS) under micellar conditions at pH 6.4. The methodology is directly applicable to the analysis of samples solubilized via sulfitolysis in the presence of SDS, and offers superior resolution in comparison with chromatography in the absence of the surfactant. Using this method, acceptable precision (day-to-day R.S.D. = 4.9%), accuracy, selectivity, range, linearity and ruggedness were achieved.
描述了一种用于反相高效液相色谱（HPLC）测定大肠杆菌发酵液中重组甲硫氨酰天冬氨酰人生长激素（MD-HGH）的方法。该技术在pH 6.4的胶束条件下使用含有正丙醇和阴离子表面活性剂十二烷基硫酸钠（SDS）的流动相。该方法直接适用于在SDS存在下通过亚硫酸氢盐溶解的样品分析，并且与不存在表面活性剂的色谱法相比具有更高的分离度。使用该方法，获得了可接受的精密度（日间相对标准偏差=4.9%）、准确度、选择性、范围、线性和耐用性。
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