Foncea R, Varela S, Sapag-Hagar M, Lavandero S
Departament of Biochemistry and Molecular Biology, School of Chemical and Pharmaceutical Sciences, University of Chile, Santiago.
Res Commun Mol Pathol Pharmacol. 1995 Mar;87(3):253-68.
Direct and indirect evidence emphasizes the participation of classical protein kinase C (cPKC) in the development and function of the mammary gland. This work shows that there are changes not only in total cPKC activity during the lactogenic cycle, but also in the relative amounts of the soluble and particulate cPKC activities and that the time-course of these two events are not the same. The time-course of translocation from the cytoplasm to the plasma membrane suggests that the soluble and particulate forms of the enzyme may be associated with growth and differentiation of the tissue, respectively. Phosphorylation patterns also show characteristic and significant differences throughout the development of the gland. These results suggest that both total mammary cPKC activity and its subcellular forms change in accordance with the proliferative and differentiative stages of the mammary gland, and that the enzyme translocation occurs during the transition from pregnancy to lactation.
直接和间接证据均强调经典蛋白激酶C（cPKC）参与乳腺的发育和功能。这项研究表明，在泌乳周期中，不仅总cPKC活性会发生变化，可溶性和颗粒性cPKC活性的相对量也会改变，而且这两个事件的时间进程并不相同。从细胞质向质膜转位的时间进程表明，该酶的可溶性和颗粒性形式可能分别与组织的生长和分化相关。在乳腺发育过程中，磷酸化模式也呈现出特征性且显著的差异。这些结果表明，乳腺总cPKC活性及其亚细胞形式均会随着乳腺的增殖和分化阶段而变化，并且酶转位发生在从妊娠到泌乳的转变过程中。
