Dørup J, Scholz H, Maunsbach A B, Kaissling B
Department of Cell Biology, University of Aarhus, Denmark.
Exp Nephrol. 1995 May-Jun;3(3):180-92.
The effects of bafilomycin, an inhibitor of the vacuolar-type (V-type) proton pump, on nephron ultrastructure and acidification were analyzed in isolated perfused kidneys from rats with acute metabolic acidosis. Acidic intracellular compartments were labelled with the weak base DAMP, that was subsequently visualized immunocytochemically. The distribution of the proton pump was studied by immunocytochemistry. Bafilomycin inhibited urinary acidification and caused pronounced ultrastructural changes in proximal tubule cells and B-type intercalated cells (B-IC cells). Immunoreactivity for DAMP showed that bafilomycin also inhibits intracellular acidification. The distribution of the proton pump was essentially unchanged by bafilomycin in A-IC cells. In B-IC cells, immunoreactivity accumulated over studded membrane material in the apical cytoplasm. The results indicate that the V-type proton pump is important both for intracellular and for urinary acidification.
在患有急性代谢性酸中毒的大鼠离体灌注肾脏中，分析了液泡型（V型）质子泵抑制剂巴弗洛霉素对肾单位超微结构和酸化的影响。用弱碱DAMP标记酸性细胞内区室，随后通过免疫细胞化学进行观察。通过免疫细胞化学研究质子泵的分布。巴弗洛霉素抑制尿液酸化，并在近端小管细胞和B型闰细胞（B-IC细胞）中引起明显的超微结构变化。DAMP的免疫反应性表明巴弗洛霉素也抑制细胞内酸化。在A-IC细胞中，巴弗洛霉素对质子泵的分布基本没有影响。在B-IC细胞中，免疫反应性在顶端细胞质中布满颗粒的膜物质上积累。结果表明，V型质子泵对细胞内和尿液酸化都很重要。
