Batra R K, Berschneider H, Curiel D T
Department of Medicine, University of North Carolina at Chapel Hill School of Medicine, USA.
Cancer Gene Ther. 1994 Sep;1(3):185-92.
Transfer of genes to the gastrointestinal epithelium would be advantageous from investigational and therapeutic standpoints. Efficient transfer of DNA to the intestinal epithelial cells, however, has been problematic with conventional viral and nonviral vectors. As an alternative, we have utilized molecular conjugate vectors to transfer DNA to enterocytes via the receptor-mediated endocytosis pathway. We were able to achieve efficient transfection of transformed (Caco2 cells) and nontransformed gastrointestinal cells derived from neonatal piglets utilizing molecular conjugate vectors. Analysis of heterologous gene expression revealed that enterocytes could serve as a secretory cellular source of alpha 1-antitrypsin and factor IX. Transient expression of heterologous DNA persisted for up to 2 weeks following transfection. Our observations suggest that molecular conjugate vectors may represent a promising strategy in the transfer of genes to cells of the gastrointestinal tract.
从研究和治疗的角度来看，将基因转移到胃肠道上皮细胞是有益的。然而，使用传统的病毒和非病毒载体将DNA有效转移到肠道上皮细胞一直存在问题。作为一种替代方法，我们利用分子偶联载体通过受体介导的内吞途径将DNA转移到肠细胞。我们能够利用分子偶联载体实现对转化的（Caco2细胞）和源自新生仔猪的未转化胃肠道细胞的有效转染。对异源基因表达的分析表明，肠细胞可以作为α1-抗胰蛋白酶和因子IX的分泌细胞来源。转染后异源DNA的瞬时表达可持续长达2周。我们的观察结果表明，分子偶联载体可能是将基因转移到胃肠道细胞的一种有前途的策略。
