2-巯基丙酰甘氨酸调节再生大鼠肝脏中c-jun和c-Ha-ras的表达。

2-Mercaptopropionylglycine modulates the expression of c-jun and c-Ha-ras in regenerating rat liver.

作者信息

Sharma D, Radhika S, Muralidhar K, Dixit A

机构信息

Department of Zoology, University of Delhi, India.

出版信息

Cancer Lett. 1995 Jul 13;93(2):201-5. doi: 10.1016/0304-3835(95)03810-j.

DOI:10.1016/0304-3835(95)03810-j

PMID:7621429

Abstract

Changes in the expression of two proto-oncogenes, c-jun and c-Ha-ras and their modulation by MPG were studied in regenerating rat liver. A significant increase in c-jun and c-Ha-ras mRNA levels was noted after partial hepatectomy. However, the increase in c-Ha-ras mRNA was much less when compared with c-jun mRNA levels. Metallothionein (MT-I) mRNA levels showed a gradual decrease following partial hepatectomy. However, the decrease in MT-I mRNA levels was not significant when intertime comparisons were made. MPG caused a significant reduction in the levels of c-jun and c-Ha-ras mRNA in the regenerating rat liver compared to untreated controls, but had very little effect on MT-I mRNA levels. Selective reduction in the expression of proto-oncogenes by MPG suggests that MPG may affect the cell proliferation by modulating the expression of these genes.

摘要

在再生大鼠肝脏中研究了两种原癌基因c-jun和c-Ha-ras的表达变化及其受MPG的调节作用。部分肝切除术后，c-jun和c-Ha-ras mRNA水平显著升高。然而，与c-jun mRNA水平相比，c-Ha-ras mRNA的升高幅度要小得多。金属硫蛋白（MT-I）mRNA水平在部分肝切除术后呈逐渐下降趋势。然而，进行不同时间点比较时，MT-I mRNA水平的下降并不显著。与未处理的对照组相比，MPG使再生大鼠肝脏中c-jun和c-Ha-ras mRNA水平显著降低，但对MT-I mRNA水平影响很小。MPG对原癌基因表达的选择性降低表明，MPG可能通过调节这些基因的表达来影响细胞增殖。