Helle J, Dircksen H, Eckert M, Nässel D R, Spörhase-Eichmann U, Schürmann F W
I. Zoologisches Institut, Abteilung für Zellbiologie, Universität Göttingen, Germany.
Cell Tissue Res. 1995 Jul;281(1):43-61. doi: 10.1007/BF00307957.
The morphology and position of putative neurohemal areas in the peripheral nervous system (ventral nerve cord and retrocerebral complex) of the cricket Gryllus bimaculatus are described. By using antisera to the amines dopamine, histamine, octopamine, and serotonin, and the neuropeptides crustacean cardioactive peptide, FMRFamide, leucokinin 1, and proctolin, an extensive system of varicose fibers has been detected throughout the nerves of all neuromeres, except for nerve 2 of the prothoracic ganglion. Immunoreactive varicose fibers occur mainly in a superficial position at the neurilemma, indicating neurosecretory storage and release of neuroactive compounds. The varicose fibers are projections from central or peripheral neurons that may extend over more than one segment. The peripheral fiber varicosities show segment-specific arrangements for each of the substances investigated. Immunoreactivity to histamine and octopamine is mainly found in the nerves of abdominal segments, whereas serotonin immunoreactivity is concentrated in subesophageal and terminal ganglion nerves. Immunoreactivity to FMRFamide and crustacean cardioactive peptide is widespread throughout all segments. Structures immunoreactive to leucokinin 1 are present in abdominal nerves, and proctolin immunostaining is found in the terminal ganglion and thoracic nerves. Codistribution of peripheral varicose fiber plexuses is regularly seen for amines and peptides, whereas the colocalization of substances in neurons has not been detected for any of the neuroactive compounds investigated. The varicose fiber system is regarded as complementary to the classical neurohemal organs.
描述了双斑蟋外周神经系统（腹神经索和脑后复合体）中假定的神经血器官的形态和位置。通过使用针对多巴胺、组胺、章鱼胺和5-羟色胺等胺类以及甲壳类心脏活性肽、FMRF酰胺、亮氨酸激肽1和促肠肌肽等神经肽的抗血清，在除前胸神经节神经2之外的所有神经节的神经中均检测到广泛的曲张纤维系统。免疫反应性曲张纤维主要位于神经鞘膜的浅表位置，表明神经活性化合物的神经分泌储存和释放。曲张纤维是中枢或外周神经元的投射，可能延伸超过一个节段。对于所研究的每种物质，外周纤维曲张呈现出节段特异性排列。对组胺和章鱼胺的免疫反应性主要在腹部节段的神经中发现，而5-羟色胺免疫反应性集中在咽下神经节和终神经节的神经中。对FMRF酰胺和甲壳类心脏活性肽的免疫反应性在所有节段中广泛分布。对亮氨酸激肽1有免疫反应性的结构存在于腹部神经中，促肠肌肽免疫染色见于终神经节和胸部神经。胺类和肽类的外周曲张纤维丛经常出现共分布情况，而在所研究的任何神经活性化合物中均未检测到物质在神经元中的共定位。曲张纤维系统被认为是对经典神经血器官的补充。
