Cantor J O, Cerreta J M, Keller S, Turino G M
Columbia University College of Physicians and Surgeons, New York, USA.
Exp Lung Res. 1995 May-Jun;21(3):423-36. doi: 10.3109/01902149509023717.
The study examined how lung hyaluronic acid content influences airspace enlargement in elastase-induced emphysema. To determine the effect of a decrease in hyaluronic acid, hamsters received a single intratracheal instillment of hyaluronidase 24 h prior to administration of pancreatic elastase by the same route. One week later, these animals showed significantly greater airspace enlargement than controls sequentially instilled with saline and elastase (128 vs. 100 microns; p < .05). Conversely, intratracheal administration of hyaluronic acid immediately after elastase instillment resulted in a marked decrease in airspace enlargement at 1 week compared to controls receiving elastase followed by saline (82 vs. 122 microns; p = .005). Since hyaluronic acid has no elastase inhibitory capacity, its effect may involve extracellular matrix interactions not directly related to elastic fiber breakdown. This concept is supported by the finding that animals treated with hyaluronidase and elastase showed no greater loss of lung elastin than that observed in the saline/elastase control group, despite demonstrating a marked increase in airspace enlargement. Further work is needed to determine how hyaluronic acid influences airspace enlargement and to evaluate the potential use of this substance as a treatment for emphysema.
该研究考察了肺透明质酸含量如何影响弹性蛋白酶诱导的肺气肿中的气腔扩大。为了确定透明质酸减少的影响，仓鼠在通过相同途径给予胰腺弹性蛋白酶前24小时接受一次气管内注射透明质酸酶。一周后，这些动物的气腔扩大程度明显大于依次注射生理盐水和弹性蛋白酶的对照组（128对100微米；p<0.05）。相反，与接受弹性蛋白酶后注射生理盐水的对照组相比，弹性蛋白酶注射后立即气管内注射透明质酸导致1周时气腔扩大明显减少（82对122微米；p = 0.005）。由于透明质酸没有弹性蛋白酶抑制能力，其作用可能涉及与弹性纤维分解不直接相关的细胞外基质相互作用。这一概念得到以下发现的支持：尽管注射透明质酸酶和弹性蛋白酶的动物气腔扩大明显增加，但其肺弹性蛋白的损失并不比生理盐水/弹性蛋白酶对照组更大。需要进一步开展研究以确定透明质酸如何影响气腔扩大，并评估该物质作为肺气肿治疗方法的潜在用途。
