Olive J E, De Abreu D M, Rastogi T, Andersen A A, Mittermaier A K, Beattie T L, Collins R A
Canadian Institute for Advanced Research Program in Evolutionary Biology, Department of Molecular and Medical Genetics, University of Toronto, Ontario.
EMBO J. 1995 Jul 3;14(13):3247-51. doi: 10.1002/j.1460-2075.1995.tb07327.x.
Several examples of inhibition of the function of a ribozyme or RNA-protein complex have shown that certain antibiotics can interact specifically with RNA. There are, however, few examples of antibiotics that have a positive, rather than a negative, effect on the function of an RNA. We have found that micromolar concentrations of viomycin, a basic, cyclic peptide antibiotic of the tuberactinomycin group, enhance the cleavage of a ribozyme derived from Neurospora VS RNA. Viomycin decreases by an order of magnitude the concentration of magnesium required for cleavage. It also stimulates an otherwise insignificant transcleavage reaction by enhancing interactions between RNA molecules. The ability of viomycin to enhance some RNA-mediated reactions but inhibit others, including translation and Group I intron splicing, demonstrates the potential for natural selection by small molecules during evolution in the 'RNA world' and may have broader implications with respect to ribozyme expression and activity in contemporary cells.
对核酶或RNA - 蛋白质复合物功能抑制的几个例子表明，某些抗生素可以与RNA特异性相互作用。然而，对抗生素对RNA功能有积极而非消极影响的例子却很少。我们发现，微摩尔浓度的紫霉素（一种属于结核放线菌素组的碱性环状肽抗生素）能增强源自粗糙脉孢菌VS RNA的核酶的切割作用。紫霉素使切割所需的镁离子浓度降低了一个数量级。它还通过增强RNA分子之间的相互作用来刺激原本不显著的反式切割反应。紫霉素增强一些RNA介导的反应但抑制其他反应（包括翻译和I组内含子剪接）的能力，证明了在“RNA世界”进化过程中小分子进行自然选择的潜力，并且可能对当代细胞中核酶的表达和活性具有更广泛的意义。
