Orezzoli A A, González Nicolini V, Bilinski M, Villar M J, Hökfelt T, Tramezzani J H
Instituto de Neurobiología, Buenos Aires, Argentina.
Gen Comp Endocrinol. 1995 Feb;97(2):179-87. doi: 10.1006/gcen.1995.1017.
The distribution of neurotensin (NT)-like immunoreactivity (LI) in the adrenal gland of the snake Waglerophis merremii has been examined immunohistochemically. Double staining, combining NT with tyrosine hydroxylase (TH) or calcitonin gene-related peptide (CGRP) antibodies and TH with CGRP antibodies, was also carried out. Results were analyzed by conventional and by confocal fluorescence microscopy. Immunostaining revealed a subpopulation of chromaffin cells containing NT-LI within the dorsal noradrenergic ribbon. In addition, there were some NT-immunoreactive (IR) fibers in this region. NT immunoreactivity was not present within adrenergic chromaffin cells or in cortical tissue. Double staining revealed CGRP-IR fibers innervating most of the chromaffin adrenergic cells. Within the dorsal noradrenergic ribbon, two groups of chromaffin TH-IR cells were present, one receiving a dense CGRP-IR innervation and another without contact with CGRP-IR terminals. The latter chromaffin cells displayed NT-LI. These results show, for the first time, the presence of a neuropeptide in chromaffin noradrenergic cells of a reptilian adrenal gland and open up the possibility that other peptides may also be present in these cells.
利用免疫组织化学方法研究了默氏瓦格勒蝰蛇肾上腺中神经降压素（NT）样免疫反应性（LI）的分布情况。还进行了双重染色，将NT与酪氨酸羟化酶（TH）或降钙素基因相关肽（CGRP）抗体相结合，以及将TH与CGRP抗体相结合。通过传统显微镜和共聚焦荧光显微镜对结果进行分析。免疫染色显示，在背侧去甲肾上腺素能带内，存在一群含有NT-LI的嗜铬细胞。此外，该区域还有一些NT免疫反应性（IR）纤维。肾上腺能嗜铬细胞或皮质组织内不存在NT免疫反应性。双重染色显示，CGRP-IR纤维支配着大多数嗜铬肾上腺能细胞。在背侧去甲肾上腺素能带内，存在两组嗜铬TH-IR细胞，一组接受密集的CGRP-IR神经支配，另一组不与CGRP-IR终末接触。后一组嗜铬细胞显示出NT-LI。这些结果首次表明，爬行动物肾上腺嗜铬去甲肾上腺素能细胞中存在一种神经肽，并开启了这些细胞中可能还存在其他肽的可能性。
