Shaginian I A, Gintsburg A L
Genetika. 1995 May;31(5):600-10.
Basic principles, characteristics, methodical peculiarities, and strategy of choice of PCR-mediated methods of genetic typing are discussed. Existing and potential fields of application of these methods for tackling many unsolved problems of molecular epidemiology of bacterial infections are considered. Advantages of PCR-mediated methods of genetic typing compared with other techniques used for studying genomic polymorphism are demonstrated. Possibilities for developing novel PCR-mediated methods with the aim of improving genetic typing are analyzed.
讨论了聚合酶链反应(PCR)介导的基因分型方法的基本原理、特点、方法学特性及选择策略。考虑了这些方法在解决细菌感染分子流行病学诸多未解决问题方面的现有及潜在应用领域。展示了PCR介导的基因分型方法与用于研究基因组多态性的其他技术相比的优势。分析了开发新型PCR介导方法以改进基因分型的可能性。