Riley C M, Tomlinson E, Jefferies T M, Redfern P H
J Chromatogr. 1979 Feb 21;162(2):153-61. doi: 10.1016/s0378-4347(00)81908-8.
A rapid, sensitive assay for tryptophan and some of its metabolites in urine, plasma and saliva has been developed using sodium dodecylsulphate as a pairing ion in a surfactant ion-pair high-performance liquid chromatography technique. The method is highly selective for tryptophan which is separated from its main indoleamine metabolites, 5-hydroxytryptophan, 5-hydroxytryptamine and 5-hydroxyindoleacetic acid, and from kynurenine. The usefulness of the assay has been demonstrated in plasma level and urinary excretion studies of orally administered tryptophan.
已开发出一种快速、灵敏的方法，用于检测尿液、血浆和唾液中的色氨酸及其某些代谢物。该方法采用十二烷基硫酸钠作为表面活性剂离子对高效液相色谱技术中的配对离子。该方法对色氨酸具有高度选择性，可将其与主要的吲哚胺代谢物5-羟色氨酸、5-羟色胺和5-羟吲哚乙酸以及犬尿氨酸分离。该检测方法在口服色氨酸的血浆水平和尿排泄研究中已得到验证。
