Schwechheimer K, Huang S, Cavenee W K
Institut für Neuropathologie, Universität-Gesamthochschule, Essen, Germany.
Int J Cancer. 1995 Jul 17;62(2):145-8. doi: 10.1002/ijc.2910620206.
Immunostaining using an affinity-purified rabbit polyclonal antibody against the extracellular domain of the epidermal-growth-factor receptor (EGFR) showed over-expression occurring in a fraction of tumor cells in 17 out of 18 human glioblastomas and in a majority of cells in 7 of the 18. Southern-blotting technique using a full-length EGFR cDNA probe showed a variable degree of amplification in 10 of the 17 glioblastomas, which was associated with EGFR over-expression in each case. In 2 of the glioblastomas with EGFR gene amplification, a rearrangement of the gene affecting the extracellular domain of the receptor was identified and DNA sequence analyses revealed an identical deletion-rearrangement of 801 base pairs between exons 2 to 7, resulting in an in-frame fusion of exons 1 and 8.
使用针对表皮生长因子受体（EGFR）细胞外结构域的亲和纯化兔多克隆抗体进行免疫染色显示，在18例人类胶质母细胞瘤中的17例中，一部分肿瘤细胞出现过表达，在18例中的7例中，大多数细胞出现过表达。使用全长EGFR cDNA探针的Southern印迹技术显示，17例胶质母细胞瘤中的10例有不同程度的扩增，在每种情况下，这都与EGFR过表达相关。在2例有EGFR基因扩增的胶质母细胞瘤中，鉴定出影响受体细胞外结构域的基因重排，DNA序列分析显示外显子2至7之间有801个碱基对的相同缺失重排，导致外显子1和8的读框内融合。
