Abdoun K, Mesnier-Sabin M, Baudry-Partiaoglou N, Nicolas P, Cohen P
Laboratoire de Bioactivation des Peptides, Institut Jacques-Monod, Paris, France.
J Comp Physiol B. 1995;165(2):102-9. doi: 10.1007/BF00301474.
Methanolic extracts from heads of the wax moth, Galleria mellonella L. contain several factors that stimulate oviposition of virgin females in vivo and spontaneous contractions of the oviduct in vitro of the cricket, Gryllus bimaculatus. The myotropic and egg-laying activities behaved distinctly during all steps of purification, including fractionation on reverse-phase high-performance liquid chromatography. A novel neuropeptide was isolated from Galleria mellonella. The peptide does not increase the motility of isolated cricket oviducts. Among 13 other myotropic or non-myotropic factors studied, none were found to be as potent as the new peptide in stimulating egg-laying activity. The dose-response curves of myotropic and non-myotropic factors indicate that (i) proctolin, L-glutamate, octopamine, leucokinin-VI, leucopyrokinin, ecdysone and 20-hydroxy-ecdysone, or equivalent structures, might be involved in Galleria mellonella oviposition control, and (ii) that only the novel wax moth neuropeptide has the ability to trigger egg laying in that insect. The significance of these findings is discussed.
大蜡螟（Galleria mellonella L.）头部的甲醇提取物含有多种因子，这些因子在体内能刺激未交配雌性大蜡螟产卵，在体外能刺激双斑蟋（Gryllus bimaculatus）输卵管的自发收缩。在包括反相高效液相色谱分级分离在内的所有纯化步骤中，促肌动作用和产卵活性表现各异。从大蜡螟中分离出一种新型神经肽。该肽不会增加离体蟋蟀输卵管的运动性。在研究的其他13种促肌动或非促肌动因子中，没有发现一种在刺激产卵活性方面与新肽一样有效。促肌动和非促肌动因子的剂量反应曲线表明：（i）促肠动素、L-谷氨酸、章鱼胺、亮氨酸激肽-VI、亮氨酸焦激肽、蜕皮激素和20-羟基蜕皮激素，或等效结构，可能参与大蜡螟产卵控制；（ii）只有这种新型大蜡螟神经肽有能力触发该昆虫产卵。讨论了这些发现的意义。
