Das Sarma J, Duttagupta C, Ali E, Dhar T K
Indian Statistical Institute, Calcutta, India.
J Immunol Methods. 1995 Jul 17;184(1):1-6. doi: 10.1016/0022-1759(95)00069-m.
For raising high titre and specific antibody to haptens or drugs, epsilon-aminocaproic acid modified bovine serum albumin (epsilon-ACA-BSA) was prepared for use as a carrier protein. Folic acid (FA) was coupled to epsilon-ACA-BSA, Imj.BSA and BSA for raising antibodies in rabbits. Enhancement of FA immunogenicity with FA-ACA-BSA was observed. Apart from determination of titre by indirect ELISA, dose-response behaviour and specificity of these antisera were also compared. FA-ACA-BSA antibody showed high sensitivity and specificity. Using this antibody, an ELISA method for the determination of FA was developed. The study provides a simple approach to raise highly specific and high titre antibody against small molecules.
为了产生针对半抗原或药物的高滴度和特异性抗体，制备了ε-氨基己酸修饰的牛血清白蛋白（ε-ACA-BSA）用作载体蛋白。将叶酸（FA）偶联到ε-ACA-BSA、免疫球蛋白修饰的牛血清白蛋白（Imj.BSA）和牛血清白蛋白（BSA）上，用于在兔体内产生抗体。观察到FA-ACA-BSA增强了FA的免疫原性。除了通过间接ELISA测定滴度外，还比较了这些抗血清的剂量反应行为和特异性。FA-ACA-BSA抗体显示出高灵敏度和特异性。利用该抗体，建立了一种测定FA的ELISA方法。该研究提供了一种简单的方法来产生针对小分子的高特异性和高滴度抗体。
