人嗜T淋巴细胞病毒（HTLV）II型调节蛋白线性表位的定位：一种HTLV-IIb限制性表位的鉴定

Mapping of linear epitopes of the regulatory proteins of human T cell lymphotropic virus (HTLV) type II: identification of an HTLV-IIb-restricted epitope.

作者信息

Pardi D, Coligan J E, Lal R B

机构信息

Retrovirus Diseases Branch, Centers for Disease Control and Prevention, Atlanta, Georgia 30333, USA.

出版信息

J Infect Dis. 1995 Aug;172(2):554-7. doi: 10.1093/infdis/172.2.554.

DOI:10.1093/infdis/172.2.554

PMID:7622904

Abstract

Epitope mapping analyses using synthetic peptides representing the RexII and TaxII proteins identified predominant seroreactivity to the carboxyl terminus of the HTLV-IIG12 TaxII protein (G12Tax 22-G12Tax24, amino acids [aa] 312-356). Moderate reactivity to only 1 RexII peptide (G12Rex9, aa 121-140) was found, while all other RexII and TaxII peptides exhibited minimal reactivity. Peptide G12Tax24 (aa 337-356) corresponded to the extended portion of the TaxII protein characteristic of HTLV-IIb viruses and appeared to represent an HTLV-IIb-restricted epitope. This study showed that this peptide can be used in immunoassays as a quick, simple serologic tool for assessing the minimal number of HTLV-IIb viruses present within specific populations, especially when genomic DNA is not available.

摘要

使用代表RexII和TaxII蛋白的合成肽进行的表位定位分析确定，针对HTLV-IIG12 TaxII蛋白（G12Tax 22-G12Tax24，氨基酸[aa] 312-356）的羧基末端存在主要的血清反应性。仅发现对1种RexII肽（G12Rex9，aa 121-140）有中度反应性，而所有其他RexII和TaxII肽的反应性极小。肽G12Tax24（aa 337-356）对应于HTLV-IIb病毒特有的TaxII蛋白的延伸部分，似乎代表了一个HTLV-IIb限制性表位。这项研究表明，该肽可用于免疫测定，作为一种快速、简单的血清学工具，用于评估特定人群中存在的HTLV-IIb病毒的最少数量，尤其是在无法获得基因组DNA的情况下。