Morselt M L, Lam T J, van Wuijckhuise L A, Franken P, Hartman E G, Schukken Y H
Faculteit der Diergeneeskunde, Vakgroep Bedrijfsdiergeneeskunde en Voortplanting, Utrecht.
Tijdschr Diergeneeskd. 1995;120(14):426-30.
This study evaluated the reliability of making bacteriological cultures of composite milk samples for the diagnosis of subclinical udder infections in dairy herds. Quarter samples as well as composite samples were collected from 482 cows with suspected subclinical mastitis from 69 Dutch dairy farms. The samples were used to measured somatic cell counts and to prepare bacteriological cultures. The sensitivity and specificity of the bacteriological cultures of the composite samples were compared with those of the quarter samples. The sensitivity of Staphylococcus aureus, Streptococcus uberis, Streptococcus agalactiae and Streptococcus dysgalactiae was 57.9%, 65.2%, 86.6%, and 60.3%, respectively. The specificity was higher than 98% for all four bacteria. Although information is lost when composite samples are used, composite samples are useful, especially for large dairy herds. The advantage and disadvantages of the use of composite samples are discussed.
本研究评估了用于诊断奶牛场亚临床乳房感染的复合乳样进行细菌培养的可靠性。从荷兰69个奶牛场的482头疑似亚临床乳腺炎奶牛中采集了乳区样本和复合样本。这些样本用于测量体细胞计数并制备细菌培养物。将复合样本细菌培养的敏感性和特异性与乳区样本的进行比较。金黄色葡萄球菌、乳房链球菌、无乳链球菌和停乳链球菌的敏感性分别为57.9%、65.2%、86.6%和60.3%。所有四种细菌的特异性均高于98%。虽然使用复合样本会丢失一些信息，但复合样本是有用的，特别是对于大型奶牛群。文中讨论了使用复合样本的优缺点。
