矿物纤维诱导的白细胞活化：细胞内和细胞外钙的作用。

Mineral fiber-induced leukocyte activation: the role of intra- and extracellular calcium.

作者信息

Ruotsalainen M, Naarala J, Savolainen K M

机构信息

National Public Health Institute, Department of Toxicology, Kuopio, Finland.

出版信息

Toxicol Lett. 1995 Aug;78(3):195-205. doi: 10.1016/0378-4274(95)03255-j.

DOI:10.1016/0378-4274(95)03255-j

PMID:7624890

Abstract

The role of intra- and extracellular calcium in the activation of human polymorphonuclear leukocytes (PMNL) to produce reactive oxygen metabolites (ROM) were studied by using soluble, formyl-methionyl-leucyl-phenylalanine (fMLP) or phorbol myristate acetate (PMA), or particulate stimuli, quartz or chrysotile. A calcium channel inhibitor, verapamil, attenuated only quartz-induced elevation of free intracellular calcium ([Ca2+]i) and ROM production. Likewise, ethyleneglycol-bis (aminoethyl ether) tetraacetic acid (EGTA) attenuated quartz-, chrysotile- and fMLP-induced elevation of [Ca2+]i and ROM production. It also inhibited PMA-induced ROM production. A calcium ionophore, A23187 amplified ROM production by all of these stimuli. These results suggest that both intra- and extra-cellular calcium are required for the full activation of respiratory burst by soluble and particulate stimuli in human PMNL.

摘要

通过使用可溶性的甲酰甲硫氨酰亮氨酰苯丙氨酸（fMLP）或佛波酯（PMA），或颗粒性刺激物石英或温石棉，研究了细胞内和细胞外钙在激活人多形核白细胞（PMNL）以产生活性氧代谢产物（ROM）中的作用。钙通道抑制剂维拉帕米仅减弱石英诱导的细胞内游离钙（[Ca2+]i）升高和ROM产生。同样，乙二醇双（氨基乙基醚）四乙酸（EGTA）减弱石英、温石棉和fMLP诱导的[Ca2+]i升高和ROM产生。它也抑制PMA诱导的ROM产生。钙离子载体A23187增强了所有这些刺激物诱导的ROM产生。这些结果表明，细胞内和细胞外钙都是人PMNL中可溶性和颗粒性刺激物充分激活呼吸爆发所必需的。