Streete J M, Berry D J, Clarke L A, Newbery J E
Poisons Unit, New Cross Hospital, London, United Kingdom.
Ther Drug Monit. 1995 Jun;17(3):280-6. doi: 10.1097/00007691-199506000-00012.
Analysis of desmethylmethsuximide by high-performance liquid chromatography (HPLC) is described. After adding an internal standard (IS), 200 microliters of plasma was buffered to pH 4.5 and extracted with dichloroethane. The organic solvent was then evaporated to dryness and the residue reconstituted in 100 microliters of mobile phase prior to injecting a 20 microliters aliquot onto a Hypersil 5 MOS column, which was eluted with acetonitrile/acetate buffer (pH 5.5) 36:64 vol/vol. Constituents were separated in approximately 8 min. Using this method, down to 1.0 mg/L of desmethylmethsuximide in plasma can be accurately determined. The method is suitable for therapeutic monitoring of desmethylmethsuximide in patient samples.
本文描述了采用高效液相色谱法（HPLC）分析去甲基甲琥胺的方法。加入内标（IS）后，将200微升血浆缓冲至pH 4.5，并用二氯乙烷萃取。然后将有机溶剂蒸发至干，残留物用100微升流动相复溶，再取20微升注入Hypersil 5 MOS柱，用乙腈/醋酸盐缓冲液（pH 5.5，体积比36:64）洗脱。各成分在约8分钟内分离。采用该方法，血浆中低至1.0 mg/L的去甲基甲琥胺均可准确测定。该方法适用于患者样本中去甲基甲琥胺的治疗药物监测。
