质子诱导X射线发射（PIXE）法用于肝组织多元素分析的样品采集与制备方法研究

Study of sample collection and preparation methods for multi element analysis in liver tissue by proton induced X-ray emission (PIXE).

作者信息

Meinel B, Bode J C, Koenig W, Richter F W

出版信息

J Clin Chem Clin Biochem. 1979 Jan;17(1):15-21. doi: 10.1515/cclm.1979.17.1.15.

DOI:10.1515/cclm.1979.17.1.15

Abstract

Large samples of tissue and biopsy specimen were taken from a single human liver, homogenized or paraffin-embedded, then analyzed by PIXE. Homogenates, including those of biopsies, proved to be suitable for PIXE-analysis of P, S, Ca, Mn, Fe, Cu, Zn, Se, Rb, Mo, Cd and, to a limited extent, Pb. Paraffin-embedding leads to a distinct elution of K, Rb, Cl and to a lesser elution of P, Zn, Se, Br and Mo. The contents of Ca and Sr, on the other hand, were higher in the paraffin-embedded sections. Sections of paraffin-embedded biopsies proved to be hardly suitable for PIXE-analysis.

摘要

从一个人的肝脏中采集了大量组织样本和活检标本，进行匀浆或石蜡包埋，然后用质子激发X射线发射分析（PIXE）进行分析。匀浆，包括活检组织的匀浆，被证明适用于对磷、硫、钙、锰、铁、铜、锌、硒、铷、钼、镉以及在有限程度上对铅进行PIXE分析。石蜡包埋会导致钾、铷、氯明显洗脱，磷、锌、硒、溴和钼洗脱程度较小。另一方面，石蜡包埋切片中的钙和锶含量较高。石蜡包埋活检标本的切片被证明几乎不适用于PIXE分析。