Thorup C, Kurkus J, Morsing P, Persson A E
Department of Physiology and Biophysics, Lund University, Sweden.
Acta Physiol Scand. 1995 Jan;153(1):43-9. doi: 10.1111/j.1748-1716.1995.tb09832.x.
Renal effects of acute renal denervation (DNX) were studied in anaesthetized rats. In a first series, whole kidney clearance measurements were made 120 and 240 min after unilateral DNX. At 240 min, urine production was 3.59 +/- 0.87 microL min-1 in control kidneys and 7.74 +/- 1.97 microL min-1 in denervated kidneys. The corresponding values for sodium excretion were 0.56 +/- 0.17 and 1.41 +/- 0.34 mumol min-1, potassium excretion 0.48 +/- 0.08 and 0.97 +/- 0.37 mumol min-1 and glomerular filtration rate (GFR) 0.83 +/- 0.08 and 1.05 +/- 0.16 mL min-1, respectively. In a second series, tubuloglomerular feedback (TGF) characteristics were determined with the stop-flow pressure (Psf) technique. With increasing time, the sensitivity of the TGF mechanism diminished in denervated rats, as indicated by an increased turning point (TP). TP was significantly increased 2 h after DNX from 19.1 +/- 1.13 in control to 25.9 +/- 1.10 nL min-1. TP was further increased 4 h after DNX to 37.3 +/- 3.12 nL min-1. However, the maximal TGF response to increased flow in the late proximal tubule was not altered. But, Psf was significantly higher in DNX rats than in the controls (47.4 +/- 1.01 vs. 43.0 +/- 1.53 mmHg) in spite of a lower blood pressure (107 +/- 2.9 vs. 119 +/- 2.2 mmHg). We conclude that intact renal nerves are essential for the setting of the TGF sensitivity and hence the regulation of GFR.
在麻醉大鼠中研究了急性肾去神经支配（DNX）对肾脏的影响。在第一个系列实验中，在单侧DNX后120分钟和240分钟进行全肾清除率测量。在240分钟时，对照肾脏的尿量为3.59±0.87微升/分钟，去神经支配肾脏的尿量为7.74±1.97微升/分钟。钠排泄的相应值分别为0.56±0.17和1.41±0.34微摩尔/分钟，钾排泄为0.48±0.08和0.97±0.37微摩尔/分钟，肾小球滤过率（GFR）分别为0.83±0.08和1.05±0.16毫升/分钟。在第二个系列实验中，采用停流压力（Psf）技术测定肾小管-肾小球反馈（TGF）特性。随着时间的增加，去神经支配大鼠中TGF机制的敏感性降低，表现为转折点（TP）增加。DNX后2小时，TP从对照组的19.1±1.13显著增加到25.9±1.10纳升/分钟。DNX后4小时，TP进一步增加到37.3±3.12纳升/分钟。然而，晚期近端小管中对流量增加的最大TGF反应未改变。但是，尽管血压较低（107±2.9对119±2.2毫米汞柱），DNX大鼠的Psf仍显著高于对照组（47.4±1.01对43.0±1.53毫米汞柱）。我们得出结论，完整的肾神经对于TGF敏感性的设定以及因此对GFR的调节至关重要。
