Wilcox M D, Schey K L, Busman M, Hildebrandt J D
Department of Cell and Molecular Pharmacology, Medical University of South Carolina, Charleston 29425, USA.
Biochem Biophys Res Commun. 1995 Jul 17;212(2):367-74. doi: 10.1006/bbrc.1995.1979.
The complete covalent structure of the gamma 2 subunit of bovine brain G proteins was determined by peptide mapping using matrix-assisted laser desorption ionization and electrospray ionization mass spectrometry and by partial sequencing using tandem mass spectrometry. Fragments were identified in proteolytic digests corresponding to all predicted internal sequences of gamma 2, but not for N- or C-terminal sequences. Fragments consistent with modifications of the terminal peptides were observed, however. The presence of these postulated modifications was verified by tandem mass spectrometry. These studies define the structure of the native protein and provide a basis for comparison to expressed proteins.
利用基质辅助激光解吸电离和电喷雾电离质谱进行肽图分析，并通过串联质谱进行部分测序，确定了牛脑G蛋白γ2亚基的完整共价结构。在蛋白水解消化物中鉴定出的片段对应于γ2所有预测的内部序列，但未鉴定出N端或C端序列。然而，观察到了与末端肽修饰一致的片段。通过串联质谱验证了这些假定修饰的存在。这些研究确定了天然蛋白质的结构，并为与表达蛋白进行比较提供了基础。
