Kakei N, Ichinose M, Tatematsu M, Shimizu M, Ishihama S, Yahagi N, Matsushima M, Fukamachi H, Miki K, Kurokawa K
First Department of Internal Medicine, Faculty of Medicine, University of Tokyo, Japan.
Biochem Biophys Res Commun. 1995 May 16;210(2):400-8. doi: 10.1006/bbrc.1995.1675.
The present study investigated the effect of long-term treatment with omeprazole on pepsinogen-producing cells and examined whether the selective CCK-B/gastrin receptor antagonist was able to prevent the omeprazole-induced changes, if occurred, in rat stomach. Rats were treated with omeprazole and/or the CCK-B/gastrin receptor antagonist for 28 days. As a result, omeprazole markedly reduced mucosal pepsinogen activity and its mRNA concentration in rat stomach. Morphologically, in fundic glands omeprazole drastically decreased the proportion of mature chief cells and reciprocally increased that of immature chief cells which were positive for class III mucin. These effects of omeprazole were attenuated by an addition of the CCK-B/gastrin receptor antagonist. Our results suggest that omeprazole retards the differentiation of chief cells in fundic mucosa probably through hypergastrinemia in adult rat.
本研究调查了长期使用奥美拉唑治疗对胃蛋白酶原产生细胞的影响，并检测了选择性CCK - B/胃泌素受体拮抗剂是否能够预防奥美拉唑引起的（如果发生的话）大鼠胃的变化。大鼠用奥美拉唑和/或CCK - B/胃泌素受体拮抗剂治疗28天。结果显示，奥美拉唑显著降低了大鼠胃黏膜中的胃蛋白酶原活性及其mRNA浓度。形态学上，在胃底腺中，奥美拉唑大幅降低了成熟主细胞的比例，相应地增加了对III类粘蛋白呈阳性的未成熟主细胞的比例。添加CCK - B/胃泌素受体拮抗剂可减弱奥美拉唑的这些作用。我们的结果表明，在成年大鼠中，奥美拉唑可能通过高胃泌素血症延缓胃底黏膜主细胞的分化。
