Chukwumah E I, Akinwande A I, Akinrimisi E O, Odugbemi T
Department of Biochemistry, College of Medicine, University of Lagos, Nigeria.
West Afr J Med. 1995 Jan-Mar;14(1):1-5.
Vibrio Cholerae "strain 110 Cal" from Calabar, Nigeria were grown in syncase broth. Exo-enterotoxin secreted into the medium was then isolated purified and characterized. The toxin had enterotoxic activity using the infant mouseassay of World Health Organization. The toxin also had a molecular weight of about 89,000 daltons by gel filtration through sephadex G-150 and 100,000 daltons by polyacrylamide gel electrophoresis (PAGE). The toxin exhibited two subunits with molecular weights of about 63,000 and 31,000 daltons on SDS-PAGE. The toxin was resolved through sephadex G-150 into three peaks having molecular weights of about 89,000, 44,000 and 14,000 daltons respectively. The three peaks had enterotoxic activity. Each peak contained a single protein band on PAGE and SDS-PAGE showed that peak I consisted of two subunits with molecular weights of 56,000 and 31,000 daltons and peak II and III were monomers.
来自尼日利亚卡拉巴尔的霍乱弧菌“110 Cal菌株”在同步酶肉汤中培养。然后将分泌到培养基中的外毒素进行分离、纯化和鉴定。使用世界卫生组织的幼鼠试验，该毒素具有肠毒素活性。通过葡聚糖G - 150凝胶过滤，该毒素的分子量约为89,000道尔顿，通过聚丙烯酰胺凝胶电泳（PAGE）测定为100,000道尔顿。在SDS - PAGE上，该毒素呈现出分子量约为63,000和31,000道尔顿的两个亚基。通过葡聚糖G - 150将该毒素分离为三个峰，分子量分别约为89,000、44,000和14,000道尔顿。这三个峰均具有肠毒素活性。每个峰在PAGE上含有一条单一蛋白质带，SDS - PAGE显示峰I由分子量为56,000和31,000道尔顿的两个亚基组成，峰II和峰III为单体。
