Jeanfaivre T, Chassevent A, Berruchon J, Savary L, Chrétien M F, Geslin J, Larra F, Tuchais E
Service de pneumologie, CHU, Angers, France.
Bull Cancer. 1995;82(5):364-70.
The authors determined the desoxyribonucleic acid (DNA) content of 45 small-cell lung cancers, by flow-cytometry (FC), from bronchial brushings to asses the prognostic meaning of the DNA index, the percentage of cells in DNA synthesis phase (% S) and the ploidy. Moreover, the value of this tumorous cells brushing was assessed for Flow-cytometric analysis. Bronchial brushing offers some advantages opposite to biopsies, but the low quantity of collected cells reduces the number of tumors analysable by FC. The output is low (39%) for determination of DNA index, and moreover low (22%) for determination of % S. In regard to the flow-cytometry analysis, the DNA content does not show any significant difference of survival where as the % S is related to survival, but it seems to be paradoxical in this study. Indeed, patients with small-cell lung cancer associated to high cells' percentage in DNA synthesis phase have the longest survival.
作者通过流式细胞术（FC）测定了45例小细胞肺癌支气管刷检标本中的脱氧核糖核酸（DNA）含量，以评估DNA指数、DNA合成期细胞百分比（%S）和倍性的预后意义。此外，还评估了这种肿瘤细胞刷检用于流式细胞术分析的价值。支气管刷检相对于活检具有一些优势，但收集到的细胞数量较少，减少了可通过FC分析的肿瘤数量。DNA指数测定的产出率较低（39%），而且%S测定的产出率更低（22%）。关于流式细胞术分析，DNA含量在生存率方面未显示出任何显著差异，而%S与生存率相关，但在本研究中这似乎自相矛盾。实际上，DNA合成期细胞百分比高的小细胞肺癌患者生存期最长。
